Product Instruction

Features

· Each fluorochrome will produce a characteristic emission spectrum under the excitation of the corresponding laser.

· Different from the conventional flow cytometer using dichroic mirrors and filters to split light, sFlo full spectral flow technology isbased on advanced cwDM design, which divides all the signals of fluorochrome into several narrow wavelength ranges of lightUsing MppCs as detectors to obtain the complete characteristic emision spectrum of each fluorochrome, so as to distinguishdifferent fluorochromes.

The collected optical signal is is converted into an electronic signal. Then spectral analysis will be performed based on the reference spectrum in the spectral library, so as to obtain the corresponding fluorescence intensity of each fluorochrome.

Technical Parameters

Sample Acquisition

Optics

By using 2 cylindrical lenses to achieve laser spot shaping, SFLO can reduce the laser energy loss and maximize excitation light efficiency, so as to improve the detection sensitivity.

Features

Application

Cell &Biology Example

Develop a 43-color panel for the characterization of conventional and unconventional T cell subsets, B cells, NK cells, monocytes, dendritic cells, and innate lymphocytes using full spectral flow cytometry.

Sahir F , Mateo J M , Steinhoff M , et al. Development of a 43 color panel for the characterization of conventional and unconventional T cell subsets, B cells, NK cells, monocytes, dendritic cells, and innate lymphoid cells using spectral flow cytometry[J]. Cytometry Part A.

C a n cer - a s s o cia ted fib ro b la s ts ( C A F s ) a re th e mo s t p rom in en t cell type in th e TME o f v a rio u s ma lig n a n cies , a n d th eir a b u n d a n ce is in v ers ely p ro p o rtio n a l to p a tien t s u rv iv a l. Wh eth er C A F s in h uma n ca n cers ca n d irectly a ffect C D 8 + tumo r - s p ecific T cell fu n ctio n th ro u g h tumo r - ex o g en o u s a n tig en p res en ta tio n o n MH C - I rema in s u n clear.

Tom J H, Marten V, Linda B et al. Enhanced antigen cross-presentation in human colorectal cancer-associated fibroblasts through upregulation of the lysosomal protease cathepsin S[J]. J Immunother Cancer. 2022 Mar;10(3):e003591.

Drug-associated Example

Combining Cetuximab and Ficlatuzumab to treat patients with refractory/relapsed head and neck squamous cell carcinoma (HNSCC), and evaluate the changes of immunophenotype before and after treatment.

Bauman J E, Ohr J, Gooding W E et al. Phase I Study of Ficlatuzumab and Cetuximab in Cetuximab-Resistant, Recurrent/Metastatic Head and Neck Cancer[J]. Cancers (Basel), 2020, 12: undefined.

Self-assembling peptide nanofiber vaccines may represent a novel, needle -free and adjuvant-free approach to elicit protective immunity against fungal and bacterial infections at skin and mucosal barrier surfaces, using full spectral flow cytometry to study nanoparticle vaccine immunity underlying mechanism of origin.

Si Y, Tian Q, Zhao F et al. Adjuvant-free nanofiber vaccine induces in situ lung dendritic cell activationand T17 responses[J]. Sci Adv, 2020, 6: eaba0995.

Medical-associated Example

Using full-spectrum flow cytometry, the distinct mechanisms of long-term viral control in two subjects in the placebo arm of a randomized controlled therapeutic vaccine trial were investigated.

Jana B, Feng G, Manukumar H M et al. Distinct mechanisms of long-term virologic control in two HIV-infected individuals after treatment interruption of anti-retroviral therapy[J]. Nat Med. 2021 Nov;27(11):1893-1898.

Exposure to early life trauma (ELT) is associated with higher relapse rates in patients with multiple sclerosis, mechanistic studies using C57BL/6J mice and an experimental autoimmune encephalitis (EAE) model.

Yee M K, Danish M, Sungjong O et al. Early-life-trauma triggers interferon-β resistance and neurodegeneration in a multiple sclerosis model via downregulated β1-adrenergic signaling[J]. Nat Commun, 2021; 12: 105.

Tumor Immunology Example

Immune checkpoint blockade (ICB) immunotherapy prolongs overall survival in cancer patients, but response rates are low. Resistance to ICB may be due to compensatory upregulation of additional immunosuppressive molecules.

Yang M, Du W, Yi L et al. Checkpoint molecules coordinately restrain hyperactivated effector T cells in the tumor microenvironment[J]. Oncoimmunology, 2020, 9: 1708064.

The effect of lung cancer cell-specific MHCII (csMHCII) expression on tumor T cell recruitment and response to anti -PD-1 therapy was examined using two in situ immunocompetent mouse models of non -small cell lung cancer.

Johnson A M, Bullock B L, Neuwelt A J et al. Cancer Cell-Intrinsic Expression of MHC Class II Regulates the Immune Microenvironment and Response to Anti-PD-1 Therapy in Lung Adenocarcinoma[J]. J Immunol, 2020, 204: 2295-2307.