Apoptosis detection

Apoptosis is an orderly or programmed way of cell death. In the early stage, phosphatidylserine (PS) on the inside of the cell membrane migrates to the outside of the cell membrane. Annexin V is a calciumdependent phospholipid binding protein with a high binding capacity to PS. Annexin V can therefore be used as a probe to detect PS exposed
outside of the cell. Annexin V is marked with fluorescein (e.g. FITC), the PI staining rejection method is used for the double staining of the
apoptotic cell, and then the flow cytometer can be used to detect the apoptotic cell.

488-nm single laser 6-color immunophenotyping
plan

Lymphocytes are the key effector cells of the immune system, maintaining human health through immune defense, immune surveillance and immune homeostasis. Lymphocyte subsets with different functions can be distinguished and their relative proportions can be obtained by binding the monoclonal antibodies marked with fluorescent dyes to antigens on the surface of lymphocytes.

488-nm single laser 9-color immunophenotyping
plan

T cells in peripheral blood generally account for 60-80% of the total lymphocytes. T cells are a highly heterogeneous cell population that can
be classified into different categories and subpopulations. Treg is a subset of CD4+T cells with significant immunosuppressive action, accounting for 1%-2% of peripheral blood lymphocytes. In the body, Treg achieves the self-tolerance and maintain immune balance by inhibiting the immune response of other cells. Lymphocyte subsets with different functions can be distinguished and T cell subsets, function, and immune checkpoint molecules on T cells can be studied by binding the monoclonal antibodies marked with fluorescent dyes to antigens on the surface of lymphocytes.